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KMID : 0624620190520120689
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BMB Reports
2019 Volume.52 No. 12 p.689 ~ p.694
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Mitochondrial genome mutations in mesenchymal stem cells derived from human dental induced pluripotent stem cells
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Park Ju-Mi
Lee Yeon-Mi
Shin Joo-Sung
Lee Hyeon-Jeong
Son Young-Bum
Park Bong-Wook
Kim Deok-Hoon
Rho Gyu-Jin
Kang Eun-Ju
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Abstract
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Ethical and safety issues have rendered mesenchymal stem cells (MSCs) popular candidates in regenerative medicine, but their therapeutic capacity is lower than that of induced pluripotent stem cells (iPSCs). This study compared original, dental tissue-derived MSCs with re-differentiated MSCs from iPSCs (iPS-MSCs). CD marker expression in iPS-MSCs was similar to original MSCs. iPS-MSCs expressed higher in pluripotent genes, but lower levels in mesodermal genes than MSCs. In addition, iPS-MSCs did not form teratomas. All iPSCs carried mtDNA mutations; some shared with original MSCs and others not previously detected therein. Shared mutations were synonymous, while novel mutations were non-synonymous or located on RNA-encoding genes. iPS-MSCs also harbored mtDNA mutations transmitted from iPSCs. Selected iPS-MSCs displayed lower mitochondrial respiration than original MSCs. In conclusion, screening for mtDNA mutations in iPSC lines for iPS-MSCs can identify mutation-free cell lines for therapeutic applications.
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KEYWORD
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hiPSCs, iPSC-derived MSCs, Mitochondrial DNA, Mutations, Regenerative medicine
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